Additional Selective and Differential Cell Culture Media
STEMCELL Technologies EpiCult™-B Mouse Medium Kit, Size: 500 mL
EpiCult™-B (mouse) is a serum-free liquid culture medium optimized for the culture of mouse mammary luminal and myoepithelial cells. It is ideal for the culture and evaluation of mouse mammary epithelial progenitors in the mammary colony-forming unit assay when used in conjunction with an irradiated feeder layer such as NIH 3T3 cells. This medium is also used for enzymatic dissociation of mouse mammary tissue when supplemented with collagenase and hyaluronidase. Addition of Human Recombinant EGF (Catalog #78006), Human Recombinant bFGF (Catalog #78003), and Heparin Solution (Catalog #07980) is required for culturing cells.
Promocell GmbH PromoCell Myocyte Growth Medium 500 ml, ready-to-use, low serum, with Supplement Mix C-39275 attached to the flask
The PromoCell Myocyte Growth Medium is a low serum (5% V/V) medium supplemented with well chosen hormones and growth factors. It was developed for the optimal expansion and maintenance of primary cardiac myocytes in culture.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine and primary rat cardiac myocytes.| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 0.5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 2 ng / ml || Insulin (recombinant human) | 5 µg / ml |
Promocell GmbH PromoCell Endothelial Cell Basal Medium MV 2 500 ml
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Promocell GmbH PromoCell Adipocyte Nutrition Medium 500 ml, ready-to-use, low serum, with C-39438 attached to the flask
PromoCell Adipocyte Nutrition Medium is a low-serum (3% V/V) medium developed for the in vitro cultivation of mature adipocytes.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine preadipocytes/adipocytes.Components:* Nutrition Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Nutrition Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.03 ml / ml || d-Biotin | 8 μg / ml || Insulin (recombinant human) | 0.5 μg / ml || Dexamethasone | 400 ng / ml |
Promocell GmbH PromoCell Chondrocyte Growth Medium 500 ml, ready-to-use, low-serum, with C-39635 attached to the flask
PromoCell Chondrocyte Growth Medium has been developed for the in vitro cultivation of human chondrocytes. The fetal calf serum in the medium is strictly pretested for optimal performance.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for primary porcine and rat chondrocytes.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.1 ml / ml |
Promocell GmbH PromoCell Follicle Dermal Papilla Cell Growth Medium 500 ml, ready-to-use, low-serum, with C-39625 attached to the flask
PromoCell Follicle Dermal Papilla Cell Growth Medium is a low-serum medium that has been developed for the in vitro cultivation of Human Follicle Dermal Papilla Cells.The medium is optimized for primary cultures of fetal, neonatal, and adult tissue, or for the maintenance of Follicle Dermal Papilla Cells.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.04 ml / ml || Bovine Pituitary Extract | 0.004 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Renal Epithelial Cell Growth Medium 2 KIT 500 ml, Contains: C-26230, C-39605
PromoCell Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine and mouse renal epithelial cells.| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 36 ng / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 4 pg / ml || Transferrin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Endothelial Cell Basal Medium MV (prf) 500 ml, phenol red-free
Endothelial Cell Growth Medium MV is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Heparin | 90 µg / ml || Hydrocortisone | 1 µg / ml |Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2. Heparin, included in all our other Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
Promocell GmbH PromoCell Skeletal Muscle Cell Basal Medium (prf) 500 ml, phenol red-free
The PromoCell Skeletal Muscle Cell Growth Medium is a low-serum medium (5% V/V) developed for the expansion of human skeletal muscle cell cultures.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Fetuin (bovine) | 50 μg / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 10 μg / ml || Dexamethasone | 0.4 μg / ml |
Promocell GmbH PromoCell Mammary Epithelial Cell Basal Medium 500 ml
The PromoCell Mammary Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the mammary gland.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for rat and murine mammary epithelial cells as well as mamma carcinoma cell lines.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml |
Promocell GmbH PromoCell Small Airway Epithelial Cell Basal Medium (prf) 500 ml, phenol red-free
PromoCell Small Airway Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the distal respiratory system. The optimal adjustment of growth factors in this medium ensures excellent in vitro growth.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine small airway epithelial cells.| Media supplement | Final supplement concentration || Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 6.7 ng / ml || Transferrin (recombinant human) | 10 μg / ml || Retinoic Acid | 0.1 ng / ml || Bovine Serum Albumin-Fatty Acid Free (BSA-FAF) | 2.5 mg/ml |
Promocell GmbH PromoCell Mammary Epithelial Cell Growth Medium 500 ml, ready-to-use, serum-free, with C-39115 attached to the flask
The PromoCell Mammary Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the mammary gland.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for rat and murine mammary epithelial cells as well as mamma carcinoma cell lines.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml |
Promocell GmbH PromoCell Endothelial Cell Basal Medium 2 500 ml
Endothelial Cell Growth Medium 2 is a low-serum (2% V/V) medium that lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 µg / ml || Heparin | 22.5 μg / ml || Hydrocortisone | 0.2 µg / ml |For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Promocell GmbH PromoCell Keratinocyte Basal Medium 2 500 ml, calcium-free
PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.125 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.33 μg / ml || Epinephrine | 0.39 μg / ml || Transferrin (recombinant human) | 10 μg / ml || CaCl2 | 0.06 mM |